Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity
نویسندگان
چکیده
منابع مشابه
Efficient replacement of plasma membrane outer leaflet phospholipids and sphingolipids in cells with exogenous lipids.
Our understanding of membranes and membrane lipid function has lagged far behind that of nucleic acids and proteins, largely because it is difficult to manipulate cellular membrane lipid composition. To help solve this problem, we show that methyl-α-cyclodextrin (MαCD)-catalyzed lipid exchange can be used to maximally replace the sphingolipids and phospholipids in the outer leaflet of the plasm...
متن کاملPhosphatidylglucoside forms specific lipid domains on the outer leaflet of the plasma membrane.
Phosphatidylglucoside (PtdGlc) is a recently discovered unique glycophospholipid involved in granulocytic differentiation of human promyelocytic leukemia cell line HL60 and in astrocytic differentiation in developing rodent brains. Using a PtdGlc-specific monoclonal antibody in immunofluorescence and immunoelectron microscopy, we showed that PtdGlc forms distinct lipid domains on the outer leaf...
متن کاملFluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.
An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differing in their affinities for ordered versus disordered regions of the bilayer, a...
متن کاملInteraction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation
Bacteria have evolved elaborate communication strategies to co-ordinate their group activities, a process termed quorum sensing (QS). Pseudomonas aeruginosa is an opportunistic pathogen that utilizes QS for diverse activities, including disease pathogenesis. P. aeruginosa has evolved a novel communication system in which the signal molecule 2-heptyl-3-hydroxy-4-quinolone (Pseudomonas Quinolone ...
متن کاملAdventures with membrane lipids.
It is a very great honour to have been awarded the Biochemical Society’s Morton Lectureship for 1994. T o be chosen by one’s peers for an award of this stature is indeed the ultimate reward for one’s life’s work, even more so when one considers the high level of achievement reached by Professor R.A. Morton himself and by the previous awardees. This award is particularly gratifying to me in reca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2019
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0223572